Figure 1.

(A) The organs of the human body infected by Scedosporium spp. Eumycetoma is a chronic deep infection of the skin and subcutaneous tissues. (B). The standard operating procedure (SOP) for the treatment of human serum and use of the ScedLFD test. Step 1: human serum is mixed 1:2 (vol:vol) with PBS buffer containing Na₂EDTA and then heated for 3 min at 100 °C in a boiling water bath. Step 2: the heated serum is centrifuged at 16,000× g for 5 min to pellet insoluble serum proteins. Step 3: following centrifugation, the clear supernatant is mixed 1:1 (vol:vol) with LFD running buffer, 100 μL is added to the sample port of the LFD test, and after 30 min, the control (C) and test (T) line intensities are determined using a Cube reader. (C) A negative test result for unrelated yeasts (Candida and Cryptococcus) and moulds (Aspergillus, Fusarium, Mucorales) of clinical significance. Note the absence of the test (T) line but the presence of the control (C) line showing that the test has run correctly. (D) A positive test result for Scedosporium spp. detected by the ScedLFD test. The species in bold are responsible for the majority of infections in humans. Note the presence of the test (T) and control (C) lines. Figure ”Created with BioRender.com”.