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. 2024 Apr 19;14(8):847. doi: 10.3390/diagnostics14080847

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Limits of detection (LODs) in serum of ScedELISA using purified extracellular polysaccharide (EPS) antigens from S. aurantiacum isolate CBS121926, S. apiospermum isolate RMRL8353, and S. boydii isolate CBS835.96. Each data point is the mean of three replicates ± SE. The LODs for each species are indicated by asterisks (*), which show a significant (Student’s t-test [p < 0.05]) difference in absorbance values compared to matched control samples (normal unspiked serum (0.00 ng/mL serum)). The LODs of ScedELISA are ~15.6 ng/mL serum, ~62.5 ng/mL serum, and ~125 ng/mL serum for S. aurantiacum, S. apiospermum, and S. boydii, respectively.