Figure 2.

Schematic overview of the retro-MMCT approach: the diagram illustrates the main steps. 1. A donor cell line containing a normal human X chromosome (CHO/HSAX) is infected with the EnvΔR lentivirus. 2–3. Colcemid treatment induces micronucleation and after centrifugation microcells from the donor cells are obtained; 4–5. Microcells are co-cultured with the recipient iPSCs to induce EnvΔR-mediated cell–microcell fusion. 6. The resulting fused cells are selected to identify those in which the normal HSAX has been acquired.