Figure 1.

Proteomic analysis of sit4∆ vacuolar membranes. (A) Volcano plot for differentially expressed proteins in sit4∆ vs. wild-type samples. The log-transformed p-values (Student’s t-test) are plotted in the vertical axis against the log-transformed fold change on the horizontal axis. The horizontal dashed line indicates a p-value of 0.05. Red dots represent proteins that are upregulated with a 1.5-fold change threshold, whereas blue dots represent proteins that are downregulated (log2 fold change of ±0.58). Grey dots represent the unchanged proteins. Vps27 protein is identified with a red dot with a black outline. (B) Number of proteins significantly up- or downregulated in the sit4∆ vacuolar membranes. (C) GO term enrichment analysis on biological processes was conducted for proteins exhibiting statistical alterations in sit4∆ samples.