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VPS27 deletion impairs the CPY pathway in the sit4Δ mutant. (A) Immunoblotting showing complete processing of CPY in both Log and PDS (24 h after Log) phases in all strains (except in pep4Δ cells, used as control). (B) CPY protein levels were quantified using GAPDH as a loading control. Values are the mean ± SD (n = 4). (C) CPY secretion was assessed using a colony immunoblot assay. A representative image is shown (n = 3).
