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. 2024 Apr 9;13(8):655. doi: 10.3390/cells13080655

Figure 5.

Figure 5

SIT4 deletion compromises autophagy and induces the Cvt pathway at the Log phase in a Vps27-independent manner. (A) Processing of the proenzyme ApeI (prApeI) to the mature enzyme (mApeI) was analyzed at the Log and PDS (24 h after Log) phases. Hxk2 was used as a loading control. The pep4∆ cells (unable to process ApeI) were used as control. A representative blot is shown. (B) Quantification of ApeI maturation. Values are the mean ± SD (n = 4); ** p < 0.01, *** p < 0.001; one-way ANOVA. (C) Immunodetection of GFP-Atg8 and GFP in cells expressing pRS416-GFP-ATG8. Hxk2 was used as a loading control. A representative blot is shown. (D) The autophagic flux (free GFP/(GFP + GFP-Atg8) ratio) was evaluated at 24 h and 48 h after the Log phase. Values are the mean ± SD (n = 3); ** p < 0.01, **** p < 0.0001; one-way ANOVA.