Table 1.
Saccharomyces cerevisiae strains used in this study.
| Strain | Genotype | Source |
|---|---|---|
| BY4741 b,e,f | Mata, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 | EUROSCARF |
| sit4Δ b,e,f | BY4741 sit4::MXHIS3 | [34] |
| vps27∆ a,b,e,f | BY4741 vps27::KanMX4 | This study |
| sit4∆vps27∆ a,b,e,f | BY4741 sit4::MXHIS3 vps27::KanMX4 | This study |
| pep4Δ | BY4741 pep4::KanMX4 | EUROSCARF |
| pho8Δ c | BY4741 pho8::HPH | [49] |
| sit4Δpho8Δ c | BY4741 sit4::KanMX4 pho8::HPH | [49] |
| vps27Δpho8Δ c | BY4741 vps27::MXHIS3 pho8::HPH | This study |
| sit4Δvps27Δpho8Δ c | BY4741 sit4::KanMX4 vps27::MXHIS3 pho8::HPH | This study |
| BY4742 d | Matα his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0 | EUROSCARF |
| sit4Δ d | BY4742 sit4::KanMX4 | EUROSCARF |
| vps27∆ d | BY4742 vps27::MXHIS3 | This study |
| sit4∆vps27∆ d | BY4742 sit4::KanMX4 vps27::MXHIS3 | This study |
| BY4741 Rho0 | BY4741 Rho0 | [50] |
Cells harboring a YCpHAC33-Prom-VPS27-3xHA, b pRS416-GFP-ATG8, c pYX242-mtPho, d pRS416-MUP1-GFP, e pRS426-GFP-PHO8, and f pVT100U-preSU9-GFP are indicated.