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Gene transfer is inefficient with application of vector to the mucosal surface. Human airway epithelia were treated with KGF at 50 ng/ml (B and D) or basal medium (A and C) for 36 h. DA-βgal virus was then added to the apical surface. There was no gene transfer regardless of the proliferative state of the cells. Identical results were obtained with cells from three separate airway cell preparations.
