TABLE 1.
Growth and lacZ phenotypes in diploid S. cerevisiae harboring hybrid expression plasmids
| Strains containing the indicated plasmid
|
Growth on −L−T−H+3AT | lacZ phenotypea | |
|---|---|---|---|
| GAL4-DB (TRP1) | GAL4-AD (LEU2) | ||
| pVA3-1 (p53) | pTD1-1 (SV40 T-Ag) | + | Blue |
| pBD | pAD | − | White |
| pBD-239ΔNef2 | pAD | − | White |
| pBD | pAD-cDNA69 | − | White |
| pBD-239ΔNef2 | pAD-cDNA69 | + | Blue |
| pVA3-1 (p53) | pAD-cDNA69 | − | White |
| pBD-239ΔNef2 | pTD1-1 (SV40 T-Ag) | − | White |
| pBD-w.t.239Nef | pAD-cDNA69 | + | Blue |
| pBD-239ΔNef2-4 | pAD-cDNA69 | + | Blue |
| pBD-239ΔNef3 | pAD-cDNA69 | − | White |
a
S. cerevisiae Y190 and Y187 containing the two expression plasmids as indicated were mated in the presence of complete YPD medium. An aliquot of the diploid cells was plated onto −L−T−H+3AT plates as well as SD synthetic medium-agar plates lacking leucine and tryptophan (−L−T). In the case of diploid yeast cells which did not grow on −L−T−H+3AT plates, colonies from −L−T plates were used to analyze for lacZ phenotype.