FIG. 8.

Effects of phosphorylated and dephosphorylated forms of the ssD-BP on AAV second-strand DNA synthesis in in vitro replication assays. The ³²P-labeled single-stranded AAV DNA with the 3′ hairpin primer (lane 1) was used as a substrate for DNA synthesis with the Klenow fragment of E. coli DNA polymerase I and unlabeled deoxynucleoside triphosphates (lane 2) as well as in the presence of either the phosphorylated ssD-BP (lane 3) or the dephosphorylated ssD-BP (lane 4) under identical conditions. DNA samples were fractionated on a 6% polyacrylamide gel and autoradiographed as described in Materials and Methods.