Table 2.

PCR cycling conditions used to amplify mitochondrial DNA and the total reaction volumes. “N/A” is used where the temperature of the annealing step is varied depending on the primers used in each reaction.

Protocol	Year	Cycling Conditions			Total Cycles	Reaction Volume
		Denaturation	Annealing	Extension
Stoneking et al. [16]	1991	94 °C 45 s	56 °C 1 min	74 °C 1 min	30	100 $\mathsf{\mu }$L
Sullivan et al. [121]	1992	94 °C 45 s	50 °C 1 min	72 °C 3 min	32	25 $\mathsf{\mu }$L
Handt et al. [122]	1996	92 °C 50 s	N/A 50 s	72 °C 50 s	40	40 $\mathsf{\mu }$L
Berger and Parson [123]	2009	95 °C 15 s	57 °C 10 s	72 °C 20 s	39	40 $\mathsf{\mu }$L
Kim et al. [124]	2013	95 °C 20 s	55 °C 60 s	72 °C 30 s	42	25 $\mathsf{\mu }$L
Cooley [125]	2023	94 °C 20 s	56 °C 20 s	72 °C 30 s	38	40 $\mathsf{\mu }$L