TABLE 2.
Depletion of V3-specific antibodies from immune seraa
Serum | % Depletionb with
peptide:
|
|
---|---|---|
Bx08-1 | R21 | |
Bx083 years | 100 | —c |
Bx261.5 years | 98 | 94 |
Bx171.5 years | — | 96 |
Antibodies captured on V3 peptide-coated plates and thus removed from the sera were detected by ELISA. The depletion was monitored by the decrease in OD450–650 through the successive passages.
Calculated for the lowest dilution of serum tested (1/20) as 100 − [100 × (OD450–650 at last passage/OD450–650 at first passage)].
—, no binding to the V3 peptide was detected.