FIG. 5.

In vivo transduction of GFP into neurons by SIN or full-length LTR vectors. HIV-1 vectors carrying a PGK-GFP expression cassette with the full-length U3 region (A and C) or the −18 deletion construct (B and D) were concentrated by ultracentrifugation and normalized for particle content prior to injection into the corpora striata of adult rats. One month after injection, brain sections were stained for immunoreactivity to the GFP protein. Both types of vectors transduced neurons very efficiently. The SIN vector often appeared to achieve a higher level of transgene expression. A representative section close to the injection site is shown for one of six injected striata per vector. Bars in panels A and C, 2 and 0.1 mm, respectively (magnifications are the same for panels B and D, respectively).