FIG. 1.

Cdk9 and cyclin T1 protein levels are increased following activation of PBLs. PBLs were cultured in media containing 10% FBS (−) (lane 1) or with PHA at a final concentration of 1 μg/ml (lane 2), PMA at a final concentration of 1 ng/ml (lane 3), or PHA and PMA together (lane 4). After 48 h, cells were lysed and protein concentrations were determined. Equal amounts of protein were analyzed for Cdk9 or cyclin T1 (Cyc T1) levels by immunoblotting. See Materials and Methods for experimental details.