Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 12;25(8):4275. doi: 10.3390/ijms25084275

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Growth of Saccharomyces cerevisiae BJ5464-NpgA transformed with Miatrr expression vector (pXK30::Miatrr) on YPEG medium supplemented with different concentrations of glycine betaine. The strain transformed with empty vector pXK30 and the wild-type strain were taken as controls. Overnight yeast cell suspensions were diluted and spotted on yeast peptone ethanol glycerol (YPEG) medium with 20 μM, 200 μM, 2 mM and 20 mM glycine betaine added, respectively. Medium without glycine betaine was taken as control. The lateral legend shows the arrangement of two spotted identification concentrations. Arabic numerals 1, 2, 3 represent three technical repeats, and Roman numerals I, II represent two biological repeats. The picture shows that heterologous expression of Miatrr in yeast can overcome the growth inhibition caused by high concentrations of glycine betaine.