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. 2024 Apr 12;25(8):4275. doi: 10.3390/ijms25084275

Figure 5.

Figure 5

Effects of silencing Miatrr via host-derived RNA interference. (A) Miatrr expression levels in M. incognita inoculated on Arabidopsis plants of three RNAi lines (Miatrr-Ri-2, Miatrr-Ri-5, Miatrr-Ri-7), a gfp-RNAi line (GFP-Ri), and the wild type (WT) at 3 dpi, via RT-qPCR detection. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) coding gene was used as an internal control. The mean values are shown. Each bar represents the standard error (SE). Different letters indicate significant differences (p < 0.05). (B) The mean number of galls per plant at 42 dpi. Each bar represents the standard deviation (SD) in each A. thaliana line. Different letters indicate significant differences (p < 0.05). (C,D) Root galls on WT and Miatrr-Ri-2 plants, respectively. The bar represents 1 cm. (E) The mean width of worms at 42 dpi in roots of each A. thaliana line, measured after staining with acid fuchsin solution. Each bar represents SD. Different letters indicate significant differences (p < 0.05). (F,G) A common individual of M. incognita in galls of WT and Miatrr-Ri-2 plants, respectively. The bar represents 200 µm. Live nematode appears bright pink after staining with acid fuchsin solution. (H) The mean number of egg masses per plant of each A. thaliana line at 42 dpi. Each bar represents SD. Different letters indicate significant differences (p < 0.05).