Figure 4.

ADI_br potentially induced intrinsic apoptotic machinery in AGS cells. Treatment of ADI_br upregulated the expressions (bad [**** p<0.0001], bax, caspase-3, caspase-7) and increased the activity (caspase-9) of the intrinsic apoptosis-related genes. β2-microglobulin (B2M) was used as the internal control for the analysis following the delta-delta Cq method. Data presented are means of three replicate readings. Statistical analysis was conducted using one-way ANOVA implemented in GraphPad Prism 8.4.2.