Figure 5.
Flow cytometry analyses confirmed the progression of apoptosis in ADI_br-treated AGS cells. The behavior of untreated (A,B) and ADI_br-treated AGS cells (C,D) stained with Annexin V-FITC and PI was observed by flow cytometry. The cell population represented in quadrants are as follows: Q1: dead/necrotic cells, Q2: late apoptotic cells, Q3: Early apoptotic cells, and Q4: live cells. The red/orange dots correspond to areas of higher cell densities, yellow is mid-range, and green/blue indicates areas with lower cell densities. FlowJoTM was used to analyze and generate images of cell-scattering behavior.