Cytotoxicity and barrier integrity from PAH mixture exposures as measured by LDH leakage and TEER, respectively. ALI-HBECs (n = 3) were apically treated with ToxMix or AbundMix for 2, 6, 10, or 24 h in DPBS with a 1% DMSO vehicle. (A) LDH leakage after AbundMix (75%, 50%, or 10%) exposure, (B) LDH leakage after ToxMix (25%, 10%, or 1%) exposure, (C) TEER after AbundMix (75%, 50%, or 10%) exposure, and (D) TEER after ToxMix (25%, 10%, or 1%) exposure. Data points represent the average % change normalized to the vehicle control. Error bars represent the standard error of the means. Asterisks indicate 640 level of significance (* padj < 0.05; one-way ANOVA with Dunnett’s post hoc test compared to the vehicle control). ToxMix (10%) at 24 h (n = 2) only. Created with BioRender.com (accessed on 10 April 2024).