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. 2024 Apr 22;25(8):4571. doi: 10.3390/ijms25084571

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative sequencing results of APOE genotypes. The genotype calling was based on rs429358 (T>C) and rs7412 (C>T) polymorphisms. The circled nucleotide indicates the position of the nucleotide change compared to the reference sequence. Homozygous ε3/ε3 shows T/T and C/C, ε2/ε2 has T/T and T/T, ε4/ε4 has C/C and C/C, at the circled positions for rs429358 and rs7412, respectively. Likewise, heterozygous ε2/ε3 shows T/T and C/T, ε3/ε4 has T/C and C/C, ε2/ε4 has T/C and C/T, at the circled positions for rs429358 and rs7412, respectively.