FIG. 3.

Detection of the JCV genome by n-PCR amplification and Southern blot analysis of tonsillar lymphocytes and stromal cells isolated from tonsil tissue. For amplification of segments of the JCV genome regulatory region, primer pairs which amplified a 388-bp internal segment were used. Lanes marked 51 through 53 contain DNA extracted from isolated and cultured tonsillar lymphocytes and DNA extracted from stromal cells from three different donors. Positive and negative controls are similar to those in Fig. 2.