FIG. 4.

Adhesion of cells to specific matrix proteins. Isolated BS-C-1 cells were either mock infected (A through C) or infected with VV (D through F). At 3 hpi, cells were detached by depletion of Ca²⁺ and transferred to glass coverslips that had been treated with PBS (−) or serum (A and D) or with PBS containing the indicated concentrations of vitronectin (filled circles), fibronectin (open squares), collagen II (open circles), or laminin (filled triangles) (B and E). At 18 hpi the percentage of adherent cells was determined before (A, B, D, and E) or after (C and F) depletion of extracellular Ca²⁺. Data shown are means ± SEM (n = 3).