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. 2024 Mar 30;22(4):159. doi: 10.3390/md22040159

Figure 3.

Figure 3

Illustration of the CRISPR-Cas9 Mechanism for Skin Regeneration. This graphic outlines the use of CRISPR-Cas9 technology for targeted gene editing in eukaryotic cells, specifically for skin regeneration. The process begins with the Cas9 protein forming a complex with a guide RNA that is complementary to a specific gene sequence associated with skin aging. This complex then locates and binds to the target DNA sequence, where Cas9 makes a precise cut. A new DNA sequence with the desired genetic information can then be inserted at the cut site for potential therapeutic purposes, such as reversing aging effects or repairing skin damage. This advanced molecular technique is also being applied to edit the genetic code of various organisms, encompassing eukaryotic cells similar to those in humans. Specifically, in the context of combating skin aging, this method allows for precise alterations to DNA sequences, facilitating the repair or reversal of age-related genetic changes in the skin. It might also offer a tool for curing genetically based diseases [46].