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Effect of PKC cofactors on activation of the NS1O replicative functions in an entirely eukaryotic replication system. NS1P and NS1O were compared for RCR of plasmids containing the left-end active (T) or inactive (G) origin of replication by using P1-Thr plus P2-pol (containing endogenous DNA polymerases). NS1O activity was tested in either the absence or presence (+ PS) of 1.5 μg of PS. All reaction were performed in the presence of 2 mM CaCl2.
