TABLE 1.
Ratios of the viral densities obtained by TEM to the viral densities obtained by EM
| Source of sample | Viral density ratios (%)
|
||||
|---|---|---|---|---|---|
| TEM/EM-DAPI | TEM/original EM-YOPRO | TEM/modified EM-YOPRO | TEM/EM-SYBR Green I | Mean | |
| Villerest Reservoir (February 1999) | 78.33 (4.55)a | 39.28 (3.48) | 45.48 (4.68) | 54.86 (2.01) | 54.49 |
| Sep Reservoir (February 1999) | NDb | 20.45 (7.24) | 32.93 (11.78) | 32.4 (7.54) | 28.59 |
| Lake Pavin (June 1998) | 37.09 (4.59) | ND | 19.86 (3.86) | 23.05 (3.37) | 26.66 |
| Lake Pavin (April 1999) | 106.2 (9.67) | 35.77 (9.58) | 52.9 (1.50) | 64.02 (18.24) | 64.72 |
| Sewage (February 1999) | 34.85 (8.43) | ND | 9.85 (1.06) | 9.46 (3.04) | 18.05 |
| T7 phages | ND | 2.26 (0.41) | 1.97 (0.52) | 5.75 (0.80) | 3.33 |
| Mean | 64.12 | 24.44 | 27.16 | 31.59 | 36.83 |
a
The values in parentheses are standard deviations based on the data obtained for five replicates.
b
ND, not determined.