TABLE 2.
Viral concentrations in untreated samples and samples treated with DNase I and RNase A
| Protocol | Source of sample | Viral concn (107 particles/ml)
|
||
|---|---|---|---|---|
| Untreateda | DNase treated | RNase treated | ||
| Modified EM-YOPRO | Villerest Reservoir, May 1999 | 5.15 (4.53)b | 4.52 (10.06) | 3.77 (1.46) |
| Lake Pavin, May 1999 | 4.23 (9.55) | 3.34 (5.42) | 2.88 (8.13) | |
| Sep Reservoir, May 1999 | 4.47 (6.26) | 3.50 (6.29) | 3.55 (7.14) | |
| TEM | Villerest Reservoir, June 1999 | 2.64 (8.19) | 1.99 (15.18) | 2.23 (7.06) |
| Lake Pavin, June 1999 | 3.05 (6.02) | 2.66 (2.04) | 2.54 (4.18) | |
a
The values for the untreated samples are significantly higher than the values for the DNase-treated and RNase-treated samples (P < 0.05; one-way analysis of variance; Fisher's least-significant-difference test).
b
The values in parentheses are coefficients of variation based on the data obtained for three replicates.