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. 2024 Mar 22;14(4):422. doi: 10.3390/life14040422

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of the benzofuran-2-ones 6–9 on HO-1 expression and viability of differentiated SH-SY5Y cells exposed to catechol stress. (A) Real-time PCR analysis showing the time course (0–6 h) of induction of HO-1 mRNA expression under catechol stress in differentiated SH-SY5Y cells. Results are expressed as the mean ± SD of three independent experiments. Statistical significance was assessed by one-way ANOVA (***: p < 0.001). (B) Western blot analysis showing the expression of HO-1 protein in differentiated SH-SY5Y exposed or not (Ctrl) for 6 h to 10 μM catechol or to 10 μM benzofuran-2-ones (6, 7, 8 and 9). Trolox (TRX) was included as a reference antioxidant. (C) Western blot analysis showing the expression levels of HO-1 protein in differentiated SH-SY5Y exposed or not (Ctrl) for 6 h to 10 μM catechol in presence or not of 10 μM benzofuran-2-ones (6, 7, 8 and 9). Trolox (TRX) was included as a reference antioxidant. Results are expressed as the mean ± SD of three independent experiments. (D) Real-time PCR analysis showing HO-1 mRNA levels in differentiated SH-SY5Y cells exposed or not (Ctrl) for 6 h to 10 μM catechol and to 10 μM TRX or 9 in presence or not of 10 μM catechol. Results are expressed as the mean ± SD of three independent experiments. Statistical significance was assessed by one-way ANOVA (***: p < 0.001). (E) Cytofluorimetric analysis of propidium iodide (PI) staining showing necrotic differentiated SH-SY5Y cells (expressed as percentage) exposed or not (Ctrl) for 24 h to 10 μM catechol in presence or not of 10 μM benzofuran-2-ones (6, 7, 8 and 9). Trolox (TRX) was included as a reference antioxidant.