TABLE 5.
Aldoxime dehydration and nitrile-hydrolyzing activities in nitrile-degrading microorganisms when they were grown with or without inducersa
| Straina | Enzyme activity (U/liter of culture)b
|
Source or reference | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PAOx dehydration
|
PAN hydrolysis
|
PyOx dehydration
|
CyPy hydrolysis
|
||||||||||
| PAOx | PAN | None | PAOx | PAN | None | PyOx | CyPy | None | PyOx | CyPy | None | ||
| Alcaligenes faecalis IFO 13111 (ATCC 8750) | – | – | – | – | – | – | 0.76 | 0.39 | – | 0.41* | 0.65* | 0.29* | 27 |
| Brevibacterium butanicum ATCC 21196 | – | – | – | – | – | – | 0.45 | 0.20 | – | 1.30* | 0.08* | 0.09* | 27 |
| Corynebacterium alkanum ATCC 21194 | 0.23 | 0.08 | – | 4.21* | 0.83* | 0.67* | 0.51 | 0.21 | – | 1.33* | 0.66* | 0.38* | 27 |
| Corynebacterium nitrilophilus NCIMB 11594 | – | – | – | – | – | – | 0.21 | 0.13 | – | 0.35 | 1.10 | 0.06 | 18 |
| Corynebacterium sp. strain C5 TPU 6015 | 3.32 | 6.08 | – | 10.7* | 27.2* | 8.39* | – | – | – | – | – | – | 22 |
| Pseudomonas fluorescens IFO 3925 | – | – | – | – | – | – | 0.53 | 0.11 | – | 0.62* | 0.14* | 0.09* | 27 |
| Pseudomonas sp. strain K-9 TPU 7177 | 0.52 | 0.78 | – | 2.03* | 1.31* | 0.76* | 0.29 | 0.12 | – | 0.23 | 0.39 | 0.41 | 25 |
| Rhodococcus erythropolis BG-13 TPU 3220 | 0.19 | 0.14 | – | 0.40* | 0.84* | 0.55* | 0.50 | 0.75 | – | 1.47* | 0.28* | 0.10* | Asano et al. |
| Rhodococcus erythropolis BG-16 TPU 3221 | 1.71 | 1.15 | – | 1.58* | 1.53* | 0.31* | – | – | – | – | – | – | Asano et al. |
| Rhodococcus rhodochrous J-1 TPU 3311 | 2.08 | 0.17 | – | 13.4* | 10.8* | 3.81* | 0.43 | 0.11 | – | 7.52 | 1.38 | 1.73 | 3, 8, 19 |
| Rhodococcus rhodochrous NCIMB 11273 (ATCC 21197) | – | – | – | – | – | – | 0.47 | 0.16 | – | 0.54* | 0.13* | 0.04* | 11 |
| Rhodococcus sp. strain AK-32 TPU 3466 | 1.4 | 6.3 | – | 28.0* | 13.8* | 1.15* | 1.15 | 0.12 | – | 189 | 9.5 | – | 27 |
| Rhodococcus sp. strain I-9 TPU 3467 | 0.72 | 0.16 | – | 1.25 | 0.61 | 1.33 | 0.84 | 0.12 | – | 0.31 | 14.6 | 3.75 | 24 |
| Rhodococcus sp. strain N-774 TPU 3465 | 7.97 | 27.2 | – | 208* | 158* | 70.2* | 7.02 | 0.81 | – | 42.0* | 17.6* | 23.5* | 23 |
| Rhodococcus sp. strain NCIMB 11215 | – | – | – | – | – | – | 1.44 | 1.33 | – | 3.23 | 1.80 | 0.52 | 10 |
| Rhodococcus sp. strain NCIMB 11216 | – | – | – | – | – | – | 0.25 | 0.31 | – | 1.62 | 0.97 | 0.32 | 9 |
| Fusarium merismoides TG-1 TPU 4500 | 0.36 | 0.17 | – | 2.07 | 4.26 | 3.86 | 0.39 | 0.25 | – | 0.11 | 0.10 | 0.12 | 2 |
| Fusarium solani TG-2 TPU 4501 | 1.05 | 0.18 | – | 4.53 | 2.65 | 2.35 | 0.79 | 0.10 | – | 0.10 | 0.37 | 0.18 | 2 |
| Candida guilliermondii IFO 0454 | – | – | – | – | – | – | 0.11 | 0.10 | – | 0.85* | 0.10* | 0.14* | 28 |
| Rhodococcus sp. strain YH3-3c TPU 3453 | 22.4 | 4.21 | – | 288* | 89.3* | 1.17* | 2.83 | 0.08 | – | 144* | 56.3* | 0.15* | 7 |
| Bacillus sp. strain OxB-1d TPU 5563 | 12.2 | 0.91 | – | 2.59 | 4.11 | 0.94 | – | – | – | – | – | – | 12 |
a
The strains were grown with PAOx, PAN, PyOx, or CyPy or without inducers. The activity marked with an asterisk hydrolyzed PAN and CyPy accumulating PAAm and NAm, respectively.
b
None, no inducer. A dash (–) indicates that no activity was detected, under the assay conditions.
c
Isolated as PAOx degrader from soil.
d
Isolated as PyOx degrader from soil.