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Binding of gD to HIgR-expressing J1.1-2 cells. Fluorescence micrographs of J1.1-2 cells transfected with pCF18 (b and c) or pcDNA3.1 (a) are shown. Cells fixed with methanol were incubated with biotinylated recombinant gD-1(Δ290–299t) followed by Extravidin-TRITC.
