Fig 5. Palmitoylation of pORF55 is crucial for efficient virion production.

(A-D) SLK.iBAC cells containing WT ORF55, ΔORF55 or the indicated mutants were induced with Dox (1 μg/mL) and sodium butyrate (0.5 mM) for 48 h, and KSHV infectious units in the supernatants were quantified (A). Viral gene transcription was quantified by RT-qPCR (B), and WCLs were analyzed by immunoblotting (C). The intracellular viral genome copy number was determined by qPCR analysis (D). (E) The cell lines as described in Fig 5A was induced with Dox (1 μg/mL) and sodium butyrate (0.5 mM) for 72 h, and the extracellular viral genome copy number was determined by qPCR.