TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| P. fluorescens BF13 | Wild type, ferulate positive, vanillate positive | 4 |
| P. fluorescens BF13-97 | BF13 derivative, ferulate negative, vanillate negative, Kmr (TnMod-OKm) | This study |
| E. coli JM109 | recA1 endA1 gyrA96 thi-1 hsdR17 (rk− mk+) supE44 relA1 λ− Δ(lac-proAB) [F′ traD36 proAB+ lacIqZΔM15] | 34 |
| E. coli S17-1 | recA harboring the tra genes of plasmid RP4 in the chromosome, proA thi-1 | 28 |
| Plasmids | ||
| pTZ18/19R | Apr | Pharmacia |
| pLAFR3 | Broad-host-range cosmid vector derived from IncP1 plasmid pRK290; RK2 replicon λcos+ lacZα; Mob+ Tra− Tcr | 3 |
| pRK2013 | Kmr; Tra+; ColE1 replicon | 24 |
| pGEM-T Easy | Apr | Promega |
| pGV1 | pGEM-T Easy containing PCR-amplified vanAB operon | This study |
| pCC1 | pLAFR3 with an EcoRI fragment from pGV1 containing vanAB operon | This study |
| pTnMod-OKm | Used as a source of TnMod-OKm; pMB1 replicon; Tn5 tnp; RP4 oriT; Kmr | 9 |
| pP1 | Kmr; 1.4-kb PstI fragment from P. fluorescens BF13-97 chromosome containing TnMod-OKm | This study |
| pB1 | Kmr; 1.3-kb BamHI fragment from P. fluorescens BF13-97 chromosome containing TnMod-OKm | This study |
| pE1 | Kmr; 6.4-kb EcoRI fragment from P. fluorescens BF13-97 chromosome containing TnMod-OKm | This study |