FIG. 3.

Determination of a putative transcription initiation site for omp40. RNA was purified from cells of T. ferrooxidans, and the cDNA was obtained with reverse transcriptase and a primer as described in Materials and Methods. The purified DNA was tailed with dCTP and, after amplification by PCR, was used for nucleotide sequencing. The Omp40-Ext1 and Omp40-Ext2 primers were used in combination with the 5′ RACE anchored primer to generate the sequence. Lanes A, C, G, and T show the sequence ladders generated. The relevant DNA sequence is shown on the right, and the position of the possible start site is indicated with an asterisk.