Table 3.
Gram-positive CRB water isolates identified and characterized in this study.
| Isolate a | Closest Species Identified by BLAST of 16S rRNA Gene | CarbaNP c | Inhibition Zone (Diameter in mm) b | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| MEM | IMI | ERT | DOR | AMP | AMC | CIP | TET | VAN | |||
| C-M4-1 | Paenibacillus lautus | − | 5 | 25 | 0 | 12.3 | 23.3 | 26.7 | 22.3 | 34.3 | 26.3 |
| K-M2-1 | Enterococcus lactis | − | 2.7 | 23.7 | 2.3 | 11.7 | 25.7 | 31.3 | 23.6 | 30 | 24.3 |
| K-M2-2 | Enterococcus faecium | − | 2 | 24.3 | 0 | 9.6 | 21 | 29 | 24 | 29.3 | 24.8 |
| K-M2-3 | Enterococcus faecium | − | 4.7 | 22.7 | 4.7 | 9.7 | 21.7 | 27.3 | 23.3 | 27.7 | 24 |
| K-M2-4 | Enterococcus faecium | − | 0 | 20.3 | 0 | 6.7 | 19.3 | 27.3 | 23.3 | 30.7 | 25.3 |
| LAR-M2-1 | Enterococcus hirae | − | 5.5 | 24.7 | 8.3 | 10.7 | 22 | 27 | 24 | 26 | 22.5 |
| CP-M2-6 | Enterococcus hirae | − | 11 | 28 | 13 | 16.2 | 29.3 | 32 | 23 | 2.8 | 22.3 |
| RL-M2-3 | Enterococcus lactis | − | 14 | 27 | 11.7 | 11.3 | 25.7 | 27.3 | 25 | 30.3 | 25.3 |
a Isolate number: in regular font, isolate obtained directly on MacConkey-meropenem medium; in bold and italics, isolates enriched in BLCVM9-meropenem medium before streaking in Mueller–Hinton-meropenem medium. Intials(s) before the first dash indicates the isolation location (Table 1). b Resistant (green), Susceptible (purple), based on the CLSI [48] and EUCAST zone of inhibition diameter breakpoint values [55]. For genera that did not have diameter data in the CLSI manual, Enterobacteriaceae cut-offs were used. MEM (meropenem), IMI (imipenem), ERT (ertapenem), DOR (doripenem), AMP (ampicillin), AMC (amoxicillin plus clavulanic acid), CIP (ciprofloxacin), TET (tetracycline), VAN (vancomycin). c “−” indicate a negative result for the CarbaNP test.