Table 4.
Detection of Carbapenemases Production by CarbaNP, mCIM, and eCIM assays, and identification of carbapenemase genes by PCR and Sequencing.
| Isolate | Closest Species Identified by 16S rRNA Gene | CarbaNP | mCIM | eCIM | Carbapenemase Gene (% DNA Identity) | Carbapenemase (% Amino Acid Similarity) |
|---|---|---|---|---|---|---|
| SS-M2-3 | Aeromonas veronii | + | + | + | Unknown | Unknown |
| BBL-M2-1 | Aeromonas veronii | + | + | + | Unknown | Unknown |
| BBL-M2-2 | Stenotrophomonas maltophilia | + | N/D | N/D | blaL1 (88.6%) | L1 (96.9%) |
| BBL-M2-3 | Aeromonas veronii | + | + | + | Unknown | Unknown |
| MRP-M2-1 | Stenotrophomonas maltophilia | + | N/D | N/D | blaL1 (87.7%) | L1 (96.0%) |
| WL-M16-1 | Enterobacter asburiae | + | N/D | N/D | blaIMI-2 (99.2%) | IMI-2 (98.6%) |
| WL-M16-2 | Enterobacter asburiae | + | N/D | N/D | blaIMI-2 (98.3%) | IMI-2 (97.7%) |
| WL-M16-3 | Enterobacter asburiae | + | N/D | N/D | blaIMI-2 (99.8%) | IMI-2 (100%) |
| WL-M16-4 | Stenotrophomonas maltophilia | + | N/D | N/D | blaL1 (88.2%) | L1 (97.0%) |
Note: Unknown indicates the inability to identify the carbapenemase gene using targeted PCR with blaCphA-, blaImiS-, or blaVIM-2-specific primers. N/D: Not Determined (for carbapenemases directly identified by PCR and sequencing). % DNA identify or amino acid similarity compared to the reference sequences blaL1 (NG_047502) and blaIMI-2 (DQ173429). “+” Indicates a positive result for that test.