Table 3.
Validation parameters of analytical method for crocetin quantification (results are expressed in ng eq. trans-crocetin/mL of serum).
| Linearity and Sensitivity | |||||
|---|---|---|---|---|---|
| Linear Equation | Regression Coefficient (r2) |
LOD (ng/mL) |
LOQ (ng/mL) |
||
| y = 0.241 x − 0.031 | 0.9988 | 0.09 | 0.3 | ||
| Precision and accuracy | |||||
| Nominal concentration (ng/mL) | Concentration found (ng/mL) | Precision (%) | Accuracy (%) | ||
| Intra-day | Inter-day | Intra-day | Inter-day | ||
| 3.0 | 3.1 +/− 0.2 | 2.3 | 5.7 | −0.5 to 13.4 | 6.0 |
| 16.0 | 15.1 +/− 0.6 | 3.4 | 4.1 | −3.5 to −8.9 | −5.6 |
| 29.0 | 27.0 +/− 0.8 | 2.7 | 3.1 | −5.2 to −10.0 | −7.9 |
| Recovery and matrix effect | |||||
| Recovery | Matrix effect | ||||
| Nominal concentration (ng/mL) | Mean +/− SD | RSD | Nominal concentration (ng/mL) | Mean +/− SD | RSD |
| 3.0 | 106.0 +/− 6.0 | 5.7 | 1.4 | 93.4 ± 2.6 | 2.8 |
| 16.0 | 94.4 +/− 3.8 | 4.1 | 14.0 | 96.6 ± 1.1 | 1.1 |
| 29.0 | 92.1 +/− 2.9 | 3.1 | |||
| Stability | |||||
| Conditions | Spiked concentration (ng/mL) | Measured concentration (ng/mL) |
Precision
(RSD %) |
Accuracy (RE %) | |
| Temperature (°C) | Time | ||||
| Room Temperature | 4 h | 14 | 13.2 ± 0.1 | 0.5% | −8.5% |
| 4 | 24 h | 14.3 ± 0.8 | 5.6% | −0.6% | |
| −20 | 1 month | 13.3 ± 0.5 | 3.6% | −7.3% | |
| 3 Freeze–thaw | 12.6 ± 0.3 | 2.0% | −12.4% | ||