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. 2024 Mar 31;14(3):866–878. doi: 10.5455/OVJ.2024.v14.i3.14

Table 2. Cycling conditions for the detection of genes in this study.

Gene Initial denaturation Denaturation Annealing Extension Final extension Cycles
Cox1 95°C
5 minutes		 95°C
20 seconds		 55°C
30 seconds		 72°C
45 seconds		 72°C
10 minutes		 40
nad1 95°C
5 minutes		 95°C
30 seconds		 51°C
30 seconds		 72 °C
45 seconds		 72°C
10 minutes		 40
actII 95°C
5 minutes		 95°C
20 seconds		 60°C
30 seconds		 72°C
75 seconds		 72°C
10 minutes		 40