TABLE 1.
LDH activity after glycerol-grown Rhizopus cultures were transferred to media containing new carbon sources
| Carbon source in growth mediuma | Reductive LDH activity (pyruvate → lactate)
|
Oxidative LDH activity (lactate → pyruvate)
|
||
|---|---|---|---|---|
| Sp act (mU/mg of protein)b | Net intensity of band on zymogramc | Sp act (mU/mg of protein)b | Net intensity of band on zymogramc | |
| Glucose | 900 | 330,000 | 56 | NDd |
| Xylose | 280 | 85,000 | 34 | ND |
| Trehalose | 250 | 64,000 | 24 | ND |
| Glycerol | 130 | ND | 9 | 68,000 |
| Ethanol | 37 | ND | 18 | 200,000 |
| Lactate | 96 | ND | 20 | 120,000 |
a
Cells were induced in the new media for 16 h at 30°C.
b
Determined by spectrophotometric analysis.
c
Sum of background-subtracted pixel values for the visible band.
d
ND, not detected.