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. 2024 Apr 26;14:9651. doi: 10.1038/s41598-024-60118-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of a 3-day in vitro storage of unfertilized rainbow trout eggs in different dilutions of CF on embryonic survival at eyed stage (A). Results were normalized to the percentage of embryos reaching eyed stage after incubation of unfertilized eggs in the non-diluted CF control condition (100% CF). Five independent experiments were performed using eggs collected from 5 different rainbow trout females. Percentages of embryos reaching eyed stage (mean ± sd) superscripted with the same letter are not significantly different, Wilcoxon–Mann Whitney test (p value < 0.05). Effect on embryonic survival of a 3-day in vitro storage of unfertilized rainbow trout eggs in coelomic fluid (CF), boiled coelomic fluid (Boiled CF), ultracentrifuged coelomic fluid (Ultracentrifuged CF) or trout mineral medium (MM) (B). Results were normalized to the percentage of eggs reaching eyed stage when the fertilization was performed immediately upon eggs collection (IVF D0). Percentages of embryos reaching eyed stage (mean ± sem) superscripted with the same letter are not significantly different, Wilcoxon–Mann Whitney (p value < 0.05). Seven independent experiments were performed using eggs collected from 7 different rainbow trout females.