FIG. 4.

Infectivity of TYLCV-Sar clones in leaf discs of N. benthamiana. (A) Southern blot of total DNA extracted 6 days after inoculation. Discs inoculated with each clone were pooled for extraction. DNAs were extracted and treated as described in Fig. 2A. DNA from a tomato plant infected by TYLCV-Sar1.8mer is shown in the first lane. Genomic ssDNA and the replicative supercoiled (sc) or open circular (oc) dsDNA forms are indicated. (B) Western blot of total proteins extracted with TCA, probed with anti-TYLCV-Sar serum as described for Fig. 2B. Controls included extracts from discs inoculated with A. tumefaciens LBA4404 carrying the pBin19 vector devoid of viral sequences, a tomato plant infected by TYLCV-Sar1.8mer, and a noninoculated plant. The CP (29 kDa) is indicated.