TABLE 1.
Relevant TYLCV constructs used in this study
Construct | Description (reference) |
---|---|
Sic | |
pSic36 | Monomer in pBluescript KS+ (11) |
pSicSst | Monomer in pUC19 |
pBinSic1.8mer | 1.8-mer in pBin19 |
pBinSic2mer | Dimer in pBin19 |
pBinSic1.2mer | 1.2-mer in pBin19 |
pSicRcv | Monomer in pUC118 |
pBinSicRcv | SicRcv 1.2-mer in pBin19 |
pBinSic708m | 1.2-mer in pBin19 with the CP of SicRcv (PHE combination) |
pBinSic2025m | 1.2-mer in pBin19 with the RepC1 of SicRcv, but the CP of Sic (PQE combination) |
Sar | |
pBinSarQQD | 1.2-mer wild type in pBin19 |
pBinSarQQE | 1.2-mer with SpeI-SnaBI fragment from pSic36 |
pBinSarPQD | 1.2-mer with SstII-SpeI fragment from pSic36 |
pBinSarPQE | 1.2-mer with SstII-SnaBI fragment from pSic36 |
pBinSarPHD | 1.2-mer with SstII-SpeI fragment from pSicRcv |
pBinSarPHE | 1.2-mer with SstII-SnaBI fragment from pSicRcv |
pBinSarQHD | 1.2-mer mutated with primers SARMUT692(+) and SARMUT725(−) |
pBinSarQHE | pBinSarQHD with SpeI-SnaBI fragment from pSicRcv |
pSar1.8mer | 1.8-mer wild type in pBin19 (22) |