Table 1.

Inhibition of hemolysis exerted through wild-type PLY by pore-blocking agents PB-1 to PB-3 and derivatives (distinct samples n ≥ 3)

Compound	Structure	IC50 [µM]a (95% CI)
PB-1		38 ± 6
PB-2		4.8 ± 0.8
PB-2.1		Inactive
PB-2.2		Inactiveb
PB-2.3		Inactive
PB-2.4		Inactive
PB-2.5		13 ± 1.7
PB-2.12		51 ± 1.6
PB-3		3.1 ± 0.5 (0.22 ± 0.09)c

^aIncubation with PLY for 10 min, for details see “Methods” section.

^bNo activity under standard conditions; prolonged incubation for 1.5 h yielded an IC₅₀ value of 18 µM.

^cWith 2.5% human serum. For extended SAR with 46 further derivatives, see Supplementary Figs. 7, 8, and 14.