Table 2.
Variability intra-assay. The coefficient of variation (CV%) indicates the variability in the assays with a different number of eggs. Values represent quintupled samples of egg homogenates by experiment. (NI) non-infected eggs; (DENV2) DENV2 infected eggs.
| Experiments | Mean OD492 | SD | Eggs NI (CV%) | Mean OD492 | SD | Eggs DENV2 (CV%) |
|---|---|---|---|---|---|---|
| 300 eggs | ||||||
| 1 | 0.1111 | 0.0094 | 8.49 | 0.1222 | 0.0149 | 12.24 |
| 2 | 0.1906 | 0.0137 | 7.21 | 0.2605 | 0.0150 | 5.77 |
| 400 eggs | ||||||
| 1 | 0.2076 | 0.0063 | 3.07 | 0.3495 | 0.0148 | 4.24 |
| 2 | 0.2348 | 0.0058 | 2.49 | 0.3206 | 0.0089 | 2.78 |
| 3 | 0.2018 | 0.0050 | 2.49 | 0.3353 | 0.0261 | 7.81 |
| 1037–1215 eggs | ||||||
| I (1037) | 0.3602 | 0.0099 | 2.75 | 0.4880 | 0.0474 | 9.71 |
| II (1069) | 0.2924 | 0.0132 | 4.52 | 0.4444 | 0.0115 | 2.60 |
| III (1215) | 0.2802 | 0.0370 | 13.22 | 0.3988 | 0.0467 | 11.71 |