TABLE 3.
Analysis of P4 properties
| Plasmid(s) and temp (°C) | Protein(s) produceda | ddATPase activityb | Aggrega-tivity of P4c | Multi-mericity of free P4 |
|---|---|---|---|---|
| pLM662 | ||||
| 18 | P1, P2, P4*, P7 | 0 | 0 | NDd |
| 28 | 0 | +, ++ | ND | |
| pLM1174, -1188, -1223, -1230, -1233, -1242, -1261, -1272 | P1, P2, P4*, P7 | 0 | ++ | ND |
| pLM1224 | P1, P2, P4*, P7 | + | 0 | + |
| pAP6 | P1, P2, P7 | 0 | ND | ND |
| pLM450 | P1, P2, P4, P7 | + | ND | ND |
| pLM743 | P4* | 0 | ND | 0 |
| pLM745 | ||||
| 18 | P4* | + | 0 | + |
| 28 | + | + | + | |
| pJTJ7.3/3, -7.3/7, -7.4/6, -7.5/7 | ΔP4 | + | 0e | +e |
| pJTJ7.5/11, pJTJ7.6/7 | ΔP4 | 0 | +e | 0e |
| pAP2 | wt P4 | + | 0 | + |
a
P4*, mutated P4; ΔP4, truncated P4.
b
All the mutant and wt P4 cell extracts were analyzed as described in the legend to Fig. 2. Symbols: 0, activity at the same level as that in the control cell extract; +, activity at the same level as that in the wt P4-containing cell extract. No intermediate activities were found.
c
The relative amount of aggregative P4 was approximated visually for the centrifugation supernatant and pellet by using Western blots. Symbols: ++, ≥90% in aggregate; +, 50% in aggregate; 0, ≤10% in aggregate.
d
ND, not determined.
e
These results have been published previously (19).