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Evaluation of the membrane permeability of the trypomastigotes after treatment with fraction II from the M. zeaxanthinifaciens EtOAc extract (FII), using the SYTOX Green dye probe and a spectrofluorometer. The untreated parasites (the control group) and those treated with 0.5% (v/v) of Triton X-100 were evaluated as the minimal and maximal permeabilization, respectively. *** p < 0.0001; and ** p< 0.001, * p < 0.05.
