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. 2024 Apr 15;16(8):1177. doi: 10.3390/nu16081177

Figure 1.

Figure 1

Vitamin A status and EMT markers in the lung. (A) RT-qPCR was performed to analyze the mRNA expression of EMT markers Cdh1, Cdh2, and β-catenin in control rats, vitamin A-deficient rats (VAD), and vitamin A-deficient rats treated with retinoic acid (RA). The data used in the statistical analyses and histograms are the mean ± SD (n = 4–5 for each group). (B) Protein levels of different EMT markers (E-Cadherin, N-Cadherin, and β-catenin) were studied by Western blot in lungs from control (C), vitamin A-deficient (VAD), and vitamin A-deficient rats treated with RA (VAD + RA). (C) Graphs showing the Western blot quantification. Data (n ≥ 3) were quantified, normalized with β-actin, and plotted as mean fold ± SD vs. control. (A,C) significant results are shown * p ≤ 0.05; ** p ≤ 0.01 *** p ≤ 0.001 or **** p ≤ 0.0001.