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. 2024 Apr 15;16(8):1177. doi: 10.3390/nu16081177

Figure 5.

Figure 5

RARα ChIP assay on furin promoter. (A) Potential binding sites for RAR and/or RXR nuclear receptors in the furine promoter region. CTF1, CTF2, and Enhancer (ENH) indicate 3 regions where most transcriptional factor binding sites that modulate gene transcription are concentrated. (B) ChIP data analysis of RARα binding to furin and MMP-9 genes in control rats (RA−) and control rats injected intraperitoneally with retinoic acid (RA+) (left panel, CONTROL group). The same analysis was carried out in vitamin A-deficient rats (RA−) and vitamin A-deficient rats injected intraperitoneally with retinoic acid (RA+) (Right panel, VAD). Chromatin was immunoprecipitated with an anti-RARα antibody (black bars) or a nonrelated antibody anti-IgG as a negative control (grey bars). Data are the result of three independent experiments and bars represent mean ± SD.