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Far-UV CD (a) and fluorescence (b) spectra of purified VP23His. Purified VP23His at 0.2 mg/ml in 20 mM sodium phosphate buffer (pH 7.5)–0.004% Nonidet P-40 was examined by far-UV CD and fluorescence spectroscopy as described in the text. Both traces were corrected for the effect of the buffer by subtraction of a buffer control spectrum. deg, degrees.
