FIG. 1.

Elution profile of micrococcin P₁ (A) RP-HPLC on a Eurosphor 100-C₁₈ column (250 by 8 mm; sample volume, 10 μl; flow rate, 3.5 ml/min; linear acetonitrile gradient, 0 to 80% in 30 min) of crude concentrate (fraction II). All contaminating compounds were separated at the beginning of the linear gradient (0 to 60% B). (B) Analytical separation of pooled fraction IV. The pure antibacterial compound was eluted as a distinct, symmetrical peak at about 84% of solvent B (67% acetonitrile). The arrow indicates the activity.