Skip to main content
. 2024 Apr 1;11(4):160. doi: 10.3390/vetsci11040160

Figure 1.

Figure 1

Effect of Clostridium butyricum (CB) on green-shell laying hens. (A,B) Changes in body weight and feed intake of laying hens over time. (C) Tibia and femur indexes. (D) Bending strength of tibia and femur. (E) Bone mineral density (BMD) of the tibia. (F) Tibial calcium content. (G) Tibia phosphorus content. (H) Tibial mRNA expression of receptor activator of nuclear factor kappa-Β ligand (RANKL), osteoprotegerin (OPG), bone morphogenetic protein 2 (BMP2) and runt-related transcription factor 2 (RUNX2). Con: Control group, fed basal diet; 108 CB: basal diet supplemented with 1 × 108 CFU/kg CB; 109 CB: basal diet supplemented with 1 × 109 CFU/kg CB. The data are presented as the mean ± SD. *, p < 0.05, **, p < 0.01.