Figure A3.

Alterations of metabolites in the fecal of NAFLD mice upon polyherbal extract intervention. Analysis results of non-targeted metabolomics in the feces from HF, HSL, and PD mice; n = 6. Permutation tests conducted with 999 random per mutations in the PLSDA model of HF vs. HSL group (a) and HF vs. PD group (b). (c,d) Principal component analysis (PCA) plot. (e) The number of significantly changed metabolites in the feces of HF mice compared to PD mice. (f) Heatmap showing the top 20 increased and decreased metabolites (as indicated by Log₂FC) in fecal extract from mice of HF group compared to mice of PD group. Significantly different metabolites were screened out by PLS-DA model (VIP > 1 and p < 0.05).